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TECHNOLOGY

Lateral Flow Assay

The Chexel™ measurement system utilizes the immunofluorescent method to measure a target analyte (for example, troponin I) in human blood. The test is automatically performed by inserting a cartridge into the immunofluorescent device, instilling a sample, and pressing the scan button. The Chexel™ cartridge consists of a sample injection port and a window through which the signals generated by the antigen-fluorescent antibody binding is recorded. The conjugation pad of the cartridge contains troponin I-specific antibody labeled by a fluorophore. Such antibodies bind with troponin I, and thus forming complexes migrate onto the nitrocellulose membrane, and are captured by antibodies on the test line. The fluorescence signal is proportional to the troponin I concentration in the sample. A sample (whole blood, plasma, or serum) is loaded through the sample injection port located at one end of the cartridge, and then the cartridge is placed on the tray on the immunofluorescence device. Pressing the scan button moves the tray into the device. Once the reaction is completed, the fluorophores at the test line are excited by a laser, and the resulting fluorescence intensity is detected and quantified by the sensor to determine the troponin I level in the sample. The measured fluorescence intensity is converted to the concentration of the particular biomarker.

 

 

Chexel Troponin I Fluorescent Reader

cTnI LOQ :  0.1ng/ml < CV 10%