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TECHNOLOGY
DNA Microarray
Key characteristics Fast Detection with High Sensitivity and Selectivity
Fast hybridization kinetics is one of the key features of DNA microarrays fabricated on NB slides. Both sides of the NB slides are coated with trillions of NanoCones™, cone-shaped organic chemical compounds. The apexes of NanoCones are functionalized with a NHS (N-hydroxyl succinimidyl) or aldehyde groups for attaching probe molecules with amino groups. These NanoCones can minimize steric hindrance and electrostatic disturbance by regularly controlling lateral spacing between surface-immobilized molecules, and eventually enhance binding kinetics between probe and target molecules.
The NB9 NHS slide was compared with two market leading slides for a DNA microarray assay to detect single base mutations of hot spots of p53 tumor suppress gene. The result demonstrated that the NB9 NHS slide showed up to 10 times enhanced sensitivity and up to 400% improved selectivity than other slides. In addition to this superiority, the NB9 NHS slide allows much faster detection than others. Even 10 min hybridization time in this study is sufficient to detect target DNAs with good reliability.
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