Gene expression profiling
DNA microarrays have made a dramatic impact on gene expression profiling studies. However, this format has not been standardized until now and is still suffering from low sensitivity for RNA (or cDNA) and undesirable variation within a microarray. Therefore, the NB slide is expected to provide a gold standard DNA microarray with the highest sensitivity and reliability for gene expression profiling.
For comparison with one of the most popular competing slides, gene expression profiling studies were performed using cDNAs reverse-transcripted out of total RNA without further amplification process. As shown in the figure below, NB slide shows very high sensitivity even using 1 μg total RNA, while the competitor has many missing spots as well as lower signal intensity than the NB slide. Moreover, the NB slide maintains a very low background signal for 1 μg total RNA without any blocking agents such as BSA, but the competitor has higher background signal in spite of being treated with BSA.
Figure 1. Fluorescence images after hybridization with cDNAs reverse-transcripted from total RNA. NB and competitor slides were tested.