– NanoCones Technology Allows Gold Standard Surface for Bioassay
NanoCones technology makes possible the following main features that, when combined, offer significant advantages over existing technologies.
a) Exact control of probe spacing/density
The dendron-modified substrate offers regular spacing between probes. Well-defined assembly of dendrons over the entire plate assures homogeneity and constant probe density.
b) Mimic of solution-phase behavior
Due to minimized steric hindrance between double helixes formed upon hybridization, very high hybridization efficiency and mimicry of solution phase behavior were observed. For example, oligomeric probe DNA discriminates a single base mismatched counterpart so effectively that contrast ratio is higher than 100. Also, SPR study showed that biotin on the dendron-modified surface captures Streptavidin more effectively than any other mixed monolayer.
c) Very low nonspecific binding
The dendron-modified layer shows very low nonspecific binding of various biomolecules on surface. This feature combined with spacing ensures high sensitivity of DNA microarrays. These outstanding factors allow DNA microarrays to be fabricated on a NanoCones surface to detect complementary DNA at low concentration (50 fM) for genetic mutation, and as little as 1 μg total RNA can be assayed successfully without any amplification for gene expression profiling research.
d) Absolute monolayer
Unlike a biochip plate having 3-dimensional complexity, the self-assembly approach of the NanoCones surface produces an absolute monolayer that ensures a well-defined immobilization of biomolecules of interest.